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Mendeley readers
Chapter title |
Assay of Flippase Activity in Proteoliposomes Using Fluorescent Lipid Derivatives
|
---|---|
Chapter number | 18 |
Book title |
P-Type ATPases
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Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3179-8_18 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3178-1, 978-1-4939-3179-8
|
Authors |
Magdalena Marek, Thomas Günther-Pomorski |
Abstract |
Specific membrane proteins, termed lipid flippases, play a central role in facilitating the movement of lipids across cellular membranes. In this protocol, we describe the reconstitution of ATP-driven lipid flippases in liposomes and the analysis of their in vitro flippase activity based on the use of fluorescent lipid derivatives. Working with purified and reconstituted systems provides a well-defined experimental setup and allows to directly characterize these membrane proteins at the molecular level. |
Mendeley readers
The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Canada | 1 | 8% |
Unknown | 12 | 92% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 6 | 46% |
Unspecified | 1 | 8% |
Student > Bachelor | 1 | 8% |
Other | 1 | 8% |
Professor | 1 | 8% |
Other | 1 | 8% |
Unknown | 2 | 15% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 5 | 38% |
Biochemistry, Genetics and Molecular Biology | 3 | 23% |
Unspecified | 1 | 8% |
Computer Science | 1 | 8% |
Chemistry | 1 | 8% |
Other | 0 | 0% |
Unknown | 2 | 15% |