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Mendeley readers
Chapter title |
Antimony-Phosphomolybdate ATPase Assay
|
---|---|
Chapter number | 12 |
Book title |
P-Type ATPases
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3179-8_12 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3178-1, 978-1-4939-3179-8
|
Authors |
Gianluca Bartolommei, Francesco Tadini-Buoninsegni |
Abstract |
Hydrolytic activity is an important functional parameter of enzymes like adenosinetriphosphatases (ATPases). It is measured to test enzyme functionality, but it also provides useful information on possible inhibitory effects of molecules that interfere with the hydrolytic process. Here, we describe a molybdenum-based protocol that makes use of potassium antimony (III) oxide tartrate and may be valuable in biochemical and biomedical investigations of ATPase enzymes as well as in high-throughput drug screening. This method has been successfully applied to native and recombinant ATPases. |
Mendeley readers
The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 10 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 5 | 50% |
Student > Bachelor | 1 | 10% |
Other | 1 | 10% |
Professor | 1 | 10% |
Researcher | 1 | 10% |
Other | 0 | 0% |
Unknown | 1 | 10% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 3 | 30% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 10% |
Agricultural and Biological Sciences | 1 | 10% |
Immunology and Microbiology | 1 | 10% |
Psychology | 1 | 10% |
Other | 1 | 10% |
Unknown | 2 | 20% |