Chapter title |
ATPase Activity Measurements by an Enzyme-Coupled Spectrophotometric Assay
|
---|---|
Chapter number | 11 |
Book title |
P-Type ATPases
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3179-8_11 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3178-1, 978-1-4939-3179-8
|
Authors |
Pankaj Sehgal, Claus Olesen, Jesper V. Møller, Sehgal, Pankaj, Olesen, Claus, Møller, Jesper V. |
Abstract |
Enzymatic coupled assays are usually based on the spectrophotometric registration of changes in NADH/NAD(+) or NADPH/NADP(+) absorption at 340 nm accompanying the oxidation/reduction of reactants that by dehydrogenases and other helper enzymes are linked to the activity of the enzymatic reaction under study. The present NADH-ATP-coupled assay for ATPase activity is a seemingly somewhat complicated procedure, but in practice adaptation to performance is easily acquired. It is a more safe and elegant method than colorimetric methods, but not suitable for handling large number of samples, and also presupposes that the activity of the helper enzymes is not severely affected by the chemical environment of the sample in which it is tested. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 58 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Bachelor | 19 | 33% |
Student > Ph. D. Student | 8 | 14% |
Researcher | 6 | 10% |
Student > Doctoral Student | 3 | 5% |
Student > Master | 3 | 5% |
Other | 5 | 9% |
Unknown | 14 | 24% |
Readers by discipline | Count | As % |
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Immunology and Microbiology | 1 | 2% |
Other | 6 | 10% |
Unknown | 14 | 24% |