Chapter title |
Reconstitution of Na(+),K(+)-ATPase in Nanodiscs.
|
---|---|
Chapter number | 36 |
Book title |
P-Type ATPases
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3179-8_36 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3178-1, 978-1-4939-3179-8
|
Authors |
Gregersen, Jonas Lindholt, Fedosova, Natalya U, Nissen, Poul, Boesen, Thomas, Jonas Lindholt Gregersen, Natalya U. Fedosova, Poul Nissen, Thomas Boesen |
Abstract |
Nanodiscs are disc-shaped self-assembled lipid bilayers encircled by membrane scaffolding proteins derived from Apolipoprotein A-1 (apo A-1). They constitute a versatile tool for studying membrane proteins since reconstitution into nanodiscs allows studies of the membrane proteins in detergent-free aqueous solutions in a lipid bilayer. Here, we apply the technique to the Na(+),K(+)-ATPase (NKA) from pig kidney using Membrane Scaffolding Protein 1 D1 (MSP1D1). Contrary to other reports, the nanodiscs obtained by our protocol are built up of the native lipids originally present in the detergent solubilized sample together with the NKA. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
South Africa | 1 | 8% |
Unknown | 12 | 92% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 4 | 31% |
Student > Master | 3 | 23% |
Student > Bachelor | 2 | 15% |
Student > Ph. D. Student | 1 | 8% |
Professor | 1 | 8% |
Other | 1 | 8% |
Unknown | 1 | 8% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 4 | 31% |
Biochemistry, Genetics and Molecular Biology | 3 | 23% |
Chemistry | 3 | 23% |
Physics and Astronomy | 1 | 8% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 8% |
Other | 0 | 0% |
Unknown | 1 | 8% |