Chapter title |
Crystallization of P-type ATPases by the High Lipid–Detergent (HiLiDe) Method
|
---|---|
Chapter number | 37 |
Book title |
P-Type ATPases
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3179-8_37 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3178-1, 978-1-4939-3179-8
|
Authors |
Oleg Sitsel, Kaituo Wang, Xiangyu Liu, Pontus Gourdon, Sitsel, Oleg, Wang, Kaituo, Liu, Xiangyu, Gourdon, Pontus |
Editors |
Maike Bublitz |
Abstract |
Determining structures of membrane proteins remains a significant challenge. A technique utilizing high lipid-detergent concentrations ("HiLiDe") circumvents the major bottlenecks of current membrane protein crystallization methods. During HiLiDe, the protein-lipid-detergent ratio is varied in a controlled way in order to yield initial crystal hits, which may be subsequently optimized by variation of the crystallization conditions and/or utilizing secondary detergents. HiLiDe preserves the advantages of classical lipid-based methods, yet is compatible with both the vapor diffusion and batch crystallization techniques. The method has been applied with particular success to P-type ATPases. |
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