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Chapter title |
Two-Dimensional Crystallization of the Ca 2+ -ATPase for Electron Crystallography
|
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Chapter number | 38 |
Book title |
P-Type ATPases
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Published in |
Methods in molecular biology, January 2016
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DOI | 10.1007/978-1-4939-3179-8_38 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3178-1, 978-1-4939-3179-8
|
Authors |
John Paul Glaves, Joseph O. Primeau, Howard S. Young, Glaves, John Paul, Primeau, Joseph O., Young, Howard S. |
Abstract |
Electron crystallography of two-dimensional crystalline arrays is a powerful alternative for the structure determination of membrane proteins. The advantages offered by this technique include a native membrane environment and the ability to closely correlate function and dynamics with crystalline preparations and structural data. Herein, we provide a detailed protocol for the reconstitution and two-dimensional crystallization of the sarcoplasmic reticulum calcium pump (also known as Ca(2+)-ATPase or SERCA) and its regulatory subunits phospholamban and sarcolipin. |
Mendeley readers
The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 4 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 1 | 25% |
Researcher | 1 | 25% |
Unknown | 2 | 50% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 1 | 25% |
Chemistry | 1 | 25% |
Unknown | 2 | 50% |