| Chapter title |
A Multifunctional Envelope-Type Nano Device Containing a pH-Sensitive Cationic Lipid for Efficient Delivery of Short Interfering RNA to Hepatocytes In Vivo
|
|---|---|
| Chapter number | 7 |
| Book title |
SiRNA Delivery Methods
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3112-5_7 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3111-8, 978-1-4939-3112-5
|
| Authors |
Yusuke Sato, Hideyoshi Harashima, Michinori Kohara, Sato, Yusuke, Harashima, Hideyoshi, Kohara, Michinori |
| Abstract |
Various types of nanoparticles have been developed with the intent of efficiently delivering short interfering RNA (siRNA) to hepatocytes to date. To achieve efficient SiRNA delivery, various aspects of the delivery processes and physical properties need to be considered. We recently developed an original lipid nanoparticle, a multifunctional envelope-type nano device (MEND) containing YSK05, a pH-sensitive cationic lipid (YSK05-MEND). The YSK05-MEND with SiRNA in its formulation showed hepatocyte-specific uptake and robust gene silencing in hepatocytes after intravenous administration. Here, we describe the procedure used in the preparation and characterization method of the YSK05-MEND. |
Mendeley readers
Geographical breakdown
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|---|---|---|
| Unknown | 7 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 3 | 43% |
| Professor > Associate Professor | 1 | 14% |
| Student > Bachelor | 1 | 14% |
| Student > Master | 1 | 14% |
| Unknown | 1 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Pharmacology, Toxicology and Pharmaceutical Science | 3 | 43% |
| Agricultural and Biological Sciences | 2 | 29% |
| Immunology and Microbiology | 1 | 14% |
| Unknown | 1 | 14% |