Chapter title |
Preparation of Polyion Complex Micelles Using Block Copolymers for SiRNA Delivery
|
---|---|
Chapter number | 9 |
Book title |
SiRNA Delivery Methods
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3112-5_9 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3111-8, 978-1-4939-3112-5
|
Authors |
Hyun Jin Kim, Meng Zheng, Kanjiro Miyata, Kazunori Kataoka, Kim, Hyun Jin, Zheng, Meng, Miyata, Kanjiro, Kataoka, Kazunori |
Abstract |
Polyion complex (PIC) micelles can be prepared through the spontaneous assembly of cationic block copolymers with oppositely charged short interfering RNAs (SiRNAs). Their core-shell architectures offer a delivery platform for vulnerable SiRNA, improving their biological activities for medicinal applications such as tumor-targeted therapy. Here, we report a protocol for the preparation of SiRNA-loaded PIC micelles using a poly(ethylene glycol)-block-poly(aspartamide) derivative, providing the physicochemical criteria for well-defined micellar formulation. In addition, we describe protocols for a stability assay for SiRNA-loaded PIC micelles in the presence of serum using fluorescence correlation spectroscopy and a luciferase assay for cultured cancer cells stably expressing luciferase, thus providing the biological criteria for further medicinal applications. |
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Geographical breakdown
Country | Count | As % |
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Unknown | 11 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 2 | 18% |
Student > Master | 2 | 18% |
Student > Bachelor | 1 | 9% |
Student > Doctoral Student | 1 | 9% |
Student > Ph. D. Student | 1 | 9% |
Other | 0 | 0% |
Unknown | 4 | 36% |
Readers by discipline | Count | As % |
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Chemical Engineering | 1 | 9% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 9% |
Agricultural and Biological Sciences | 1 | 9% |
Medicine and Dentistry | 1 | 9% |
Other | 1 | 9% |
Unknown | 4 | 36% |