| Chapter title |
Delivery of Small Interfering RNAs to Cells via Exosomes.
|
|---|---|
| Chapter number | 10 |
| Book title |
SiRNA Delivery Methods
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3112-5_10 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3111-8, 978-1-4939-3112-5
|
| Authors |
Wahlgren, Jessica, Statello, Luisa, Skogberg, Gabriel, Telemo, Esbjörn, Valadi, Hadi, Jessica Wahlgren, Luisa Statello, Gabriel Skogberg, Esbjörn Telemo, Hadi Valadi |
| Abstract |
Exosomes are small membrane bound vesicles between 30 and 100 nm in diameter of endocytic origin that are secreted into the extracellular environment by many different cell types. Exosomes play a role in intercellular communication by transferring proteins, lipids, and RNAs to recipient cells.Exosomes from human cells could be used as vectors to provide cells with therapeutic RNAs. Here we describe how exogenous small interfering RNAs may successfully be introduced into various kinds of human exosomes using electroporation and subsequently delivered to recipient cells. Methods used to confirm the presence of siRNA inside exosomes and cells are presented, such as flow cytometry, confocal microscopy, and Northern blot. |
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