| Chapter title |
Synthetic SiRNA Delivery: Progress and Prospects.
|
|---|---|
| Chapter number | 23 |
| Book title |
SiRNA Delivery Methods
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3112-5_23 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3111-8, 978-1-4939-3112-5
|
| Authors |
Roberts, Thomas C, Ezzat, Kariem, El Andaloussi, Samir, Weinberg, Marc S, Thomas C. Roberts, Kariem Ezzat, Samir EL Andaloussi, Marc S. Weinberg, Roberts, Thomas C., Andaloussi, Samir, Weinberg, Marc S., Andaloussi, Samir EL |
| Abstract |
Small interfering RNA (siRNA) is a powerful tool for modulating gene expression by RNA interference (RNAi). Duplex RNA oligonucleotides induce cleavage of homologous target transcripts, thereby enabling posttranscriptional silencing of potentially any gene. As such, siRNAs may have utility as novel pharmaceuticals for a wide range of diseases. However, a lack of "drug-likeness," physiological barriers, and potential toxicities have meant that systemic delivery of SiRNAs in vivo remains a major challenge. Here we discuss various strategies that have been employed to solve the problem of SiRNA delivery. These include chemical modification of the SiRNA, direct conjugation to bioactive moieties, and nanoparticle formulations. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 50% |
| Science communicators (journalists, bloggers, editors) | 1 | 50% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 1% |
| Unknown | 80 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 16 | 20% |
| Researcher | 12 | 15% |
| Student > Master | 9 | 11% |
| Student > Bachelor | 8 | 10% |
| Student > Doctoral Student | 3 | 4% |
| Other | 11 | 14% |
| Unknown | 22 | 27% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 13 | 16% |
| Agricultural and Biological Sciences | 10 | 12% |
| Chemistry | 8 | 10% |
| Pharmacology, Toxicology and Pharmaceutical Science | 5 | 6% |
| Medicine and Dentistry | 5 | 6% |
| Other | 15 | 19% |
| Unknown | 25 | 31% |