| Chapter title |
Non-Covalently Functionalized of Single-Walled Carbon Nanotubes by DSPE-PEG-PEI for SiRNA Delivery
|
|---|---|
| Chapter number | 13 |
| Book title |
SiRNA Delivery Methods
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3112-5_13 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3111-8, 978-1-4939-3112-5
|
| Authors |
King Sun Siu, Yujuan Zhang, Xiufen Zheng, James Koropatnick, Wei-Ping Min, Siu, King Sun, Zhang, Yujuan, Zheng, Xiufen, Koropatnick, James, Min, Wei-Ping |
| Abstract |
The expression of a gene can be specifically downregulated by small interfering RNA (SiRNA). Modified carbon nanotubes (CNT) can be used to protect SiRNA and facilitate its entry into cells. Regardless of that, simple and efficient functionalization of CNT is lacking. Effective SiRNA delivery can be carried out using non-covalently functionalized CNT, where non-covalent (versus covalent) functionalization is simpler and more expeditious. Non-covalently functionalized single walled carbon nanotubes (SWCNT) that include a lipopolymer are described here. Polyethylenimine (PEI) conjugated to 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethylene glycol)-2000] (DSPE-PEG) was generated and the products used to disperse CNT to form DSPE-PEG-PEI/CNT (DGI/C), an agent capable of facilitating SiRNA delivery to cells in vitro and organs and cells in vivo. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 10 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 2 | 20% |
| Student > Master | 2 | 20% |
| Other | 1 | 10% |
| Student > Bachelor | 1 | 10% |
| Student > Ph. D. Student | 1 | 10% |
| Other | 2 | 20% |
| Unknown | 1 | 10% |
| Readers by discipline | Count | As % |
|---|---|---|
| Materials Science | 2 | 20% |
| Biochemistry, Genetics and Molecular Biology | 1 | 10% |
| Earth and Planetary Sciences | 1 | 10% |
| Agricultural and Biological Sciences | 1 | 10% |
| Chemistry | 1 | 10% |
| Other | 1 | 10% |
| Unknown | 3 | 30% |