| Chapter title |
Optogenetics
|
|---|---|
| Chapter number | 10 |
| Book title |
Optogenetics
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3512-3_10 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3510-9, 978-1-4939-3512-3
|
| Authors |
Wietek, Jonas, Prigge, Matthias, Jonas Wietek, Matthias Prigge |
| Editors |
Arash Kianianmomeni |
| Abstract |
After the discovery of Channelrhodopsin, a light-gated ion channel, only a few people saw the diverse range of applications for such a protein. Now, more than 10 years later Channelrhodopsins have become widely accepted as the ultimate tool to control the membrane potential of excitable cells via illumination. The demand for more application-specific Channelrhodopsin variants started a race between protein engineers to design improved variants. Even though many engineered variants have undisputable advantages compared to wild-type variants, many users are alienated by the tremendous amount of new variants and their perplexing names.Here, we review new variants whose efficacy has already been proven in neurophysiological experiments, or variants which are likely to extend the optogenetic toolbox. Variants are described based on their mechanistic and operational properties in terms of expression, kinetics, ion selectivity, and wavelength responsivity. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 2 | 50% |
| Germany | 1 | 25% |
| Unknown | 1 | 25% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 75% |
| Scientists | 1 | 25% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 50 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 10 | 20% |
| Researcher | 8 | 16% |
| Student > Master | 7 | 14% |
| Student > Bachelor | 6 | 12% |
| Student > Doctoral Student | 3 | 6% |
| Other | 9 | 18% |
| Unknown | 7 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Neuroscience | 13 | 26% |
| Agricultural and Biological Sciences | 9 | 18% |
| Biochemistry, Genetics and Molecular Biology | 7 | 14% |
| Physics and Astronomy | 4 | 8% |
| Psychology | 2 | 4% |
| Other | 5 | 10% |
| Unknown | 10 | 20% |