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Multipotent Stem Cells of the Hair Follicle

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Cover of 'Multipotent Stem Cells of the Hair Follicle'

Table of Contents

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    Book Overview
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    Chapter 1 Multipotent Stem Cells of the Hair Follicle
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    Chapter 2 Nestin-Based Reporter Transgenic Mouse Lines.
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    Chapter 3 Discovery of HAP Stem Cells.
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    Chapter 4 Multipotent Stem Cells of the Hair Follicle
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    Chapter 5 Construction of Tissue-Engineered Nerve Conduits Seeded with Neurons Derived from Hair-Follicle Neural Crest Stem Cells.
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    Chapter 6 Nestin-Expressing Hair-Follicle-Associated Pluripotent (HAP) Stem Cells Promote Whisker Sensory-Nerve Growth in Long-Term 3D-Gelfoam® Histoculture.
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    Chapter 7 Isolation and Culture of Neural Crest Stem Cells from Human Hair Follicles.
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    Chapter 8 Isolation of Mouse Hair Follicle Bulge Stem Cells and Their Functional Analysis in a Reconstitution Assay.
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    Chapter 9 Hair Follicle Regeneration by Transplantation of a Bioengineered Hair Follicle Germ.
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    Chapter 10 Hair Induction by Cultured Mesenchymal Cells Using Sphere Formation.
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    Chapter 11 Stereological Quantification of Cell-Cycle Kinetics and Mobilization of Epithelial Stem Cells during Wound Healing.
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    Chapter 12 Culture of Dermal Papilla Cells from Ovine Wool Follicles: An In Vitro Model for Papilla Size Determination.
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    Chapter 13 Isolation and Fluorescence-Activated Cell Sorting of Mouse Keratinocytes Expressing β-Galactosidase.
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    Chapter 14 Protocols for Ectopic Hair Growth from Transplanted Whisker Follicles on the Spinal Cord of Mice.
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    Chapter 15 Protocols for Gelfoam(®) Histoculture of Hair-Shaft-Producing Mouse Whisker Follicles Containing Nestin-GFP-Expressing Hair-Follicle-Associated Pluripotent (HAP) Stem Cells for Long Time Periods.
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    Chapter 16 Multipotent Stem Cells of the Hair Follicle
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    Chapter 17 Highly Efficient Neural Differentiation of CD34-Positive Hair-Follicle-Associated Pluripotent Stem Cells Induced by Retinoic Acid and Serum-Free Medium.
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    Chapter 18 Multipotent Stem Cells of the Hair Follicle
Attention for Chapter 18: Multipotent Stem Cells of the Hair Follicle
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Chapter title
Multipotent Stem Cells of the Hair Follicle
Chapter number 18
Book title
Multipotent Stem Cells of the Hair Follicle
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3786-8_18
Pubmed ID
Book ISBNs
978-1-4939-3784-4, 978-1-4939-3786-8
Authors

Kajiura, Satoshi, Mii, Sumiyuki, Aki, Ryoichi, Hamada, Yuko, Arakawa, Nobuko, Kawahara, Katsumasa, Li, Lingna, Katsuoka, Kensei, Hoffman, Robert M, Amoh, Yasuyuki, Satoshi Kajiura, Sumiyuki Mii, Ryoichi Aki, Yuko Hamada, Nobuko Arakawa, Katsumasa Kawahara, Lingna Li, Kensei Katsuoka, Robert M. Hoffman, Yasuyuki Amoh

Editors

Robert M Hoffman

Abstract

Hair follicles contain nestin-expressing pluripotent stem cells, the origin of which is above the bulge area, below the sebaceous gland. We have termed these cells hair-follicle-associated pluripotent (HAP) stem cells. Cryopreservation methods of the hair follicle that maintain the pluripotency of HAP stem cells are described in this chapter. Intact hair follicles from green fluorescent protein (GFP) transgenic mice were cryopreserved by slow-rate cooling in TC-Protector medium and storage in liquid nitrogen. After thawing, the upper part of the hair follicle was isolated and cultured in DMEM with fetal bovine serum (FBS). After 4 weeks culture, cells from the upper part of the hair follicles grew out. The growing cells were transferred to DMEM/F12 without FBS. After 1 week culture, the growing cells formed hair spheres, each containing approximately 1 × 10(2) HAP stem cells. The hair spheres contained cells which could differentiate to neurons, glial cells, and other cell types. The formation of hair spheres by the thawed and cultured upper part of the hair follicle produced almost as many pluripotent hair spheres as fresh follicles. The hair spheres derived from cryopreserved hair follicles were as pluripotent as hair spheres from fresh hair follicles. These results suggest that the cryopreservation of the whole hair follicle is an effective way to store HAP stem cells for personalized regenerative medicine, enabling any individual to maintain a bank of pluripotent stem cells for future clinical use.

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Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 July 2016.
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#4,275,067
of 8,085,102 outputs
Outputs from Methods in molecular biology
#1,946
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#141,653
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Outputs of similar age from Methods in molecular biology
#2
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Altmetric has tracked 8,085,102 research outputs across all sources so far. This one is in the 27th percentile – i.e., 27% of other outputs scored the same or lower than it.
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