Isolation of Mouse Hair Follicle Bulge Stem Cells and Their Functional Analysis in a Reconstitution Assay.
Multipotent Stem Cells of the Hair Follicle
Methods in molecular biology, January 2016
Ying Zheng Ph.D., Jen-Chih Hsieh Ph.D., Julia Escandon M.D., George Cotsarelis M.D., Ying Zheng, Jen-Chih Hsieh, Julia Escandon, George Cotsarelis
Robert M Hoffman
The hair follicle (HF) is a dynamic structure readily accessible within the skin, and contains various pools of stem cells that have a broad regenerative potential during normal homeostasis and in response to injury. Recent discoveries demonstrating the multipotent capabilities of hair follicle stem cells and the easy access to skin tissue make the HF an attractive source for isolating stem cells and their subsequent application in tissue engineering and regenerative medicine. Here, we describe the isolation and purification of hair follicle bulge stem cells from mouse skin, and hair reconstitution assays that allows the functional analysis of multipotent stem cells.
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