Chapter title |
Culture of Dermal Papilla Cells from Ovine Wool Follicles: An In Vitro Model for Papilla Size Determination.
|
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Chapter number | 12 |
Book title |
Multipotent Stem Cells of the Hair Follicle
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Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3786-8_12 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3784-4, 978-1-4939-3786-8
|
Authors |
Nicholas W. Rufaut, Allan J. Nixon, Rodney D. Sinclair, Rufaut, Nicholas W., Nixon, Allan J., Sinclair, Rodney D. |
Editors |
Robert M Hoffman |
Abstract |
Common human balding or hair loss is driven by follicle miniaturization. Miniaturization is thought to be caused by a reduction in dermal papilla size. The molecular mechanisms that regulate papilla size are poorly understood, and their elucidation would benefit from a tractable experimental model. We have found that dermal papilla cells from sheep spontaneously aggregate in culture to form papilla-like structures. Here, we describe methods for microdissecting dermal papillae from wool follicles, for initiating and maintaining cultures of ovine papilla cells, and for using these cells in an in vitro assay to measure the effect of bioactive molecules on aggregate size. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 3 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Professor | 1 | 33% |
Student > Postgraduate | 1 | 33% |
Unknown | 1 | 33% |
Readers by discipline | Count | As % |
---|---|---|
Nursing and Health Professions | 1 | 33% |
Agricultural and Biological Sciences | 1 | 33% |
Unknown | 1 | 33% |