| Chapter title |
Isolation and Fluorescence-Activated Cell Sorting of Mouse Keratinocytes Expressing β-Galactosidase.
|
|---|---|
| Chapter number | 13 |
| Book title |
Multipotent Stem Cells of the Hair Follicle
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3786-8_13 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3784-4, 978-1-4939-3786-8
|
| Authors |
Maria Kasper, Rune Toftgård, Viljar Jaks |
| Editors |
Robert M Hoffman |
| Abstract |
During the past decade, the rapid development of new transgenic and knock-in mouse models has propelled epidermal stem-cell research into "fast-forward mode". It has become possible to identify and visualize defined cell populations during normal tissue maintenance, and to follow their progeny during the processes of homeostasis, wound repair, and tumorigenesis. Moreover, these cells can be isolated using specific labels, and characterized in detail using an array of molecular and cell biology approaches. The bacterial enzyme, β-galactosidase (β-gal), the product of the LacZ gene, is one of the most commonly used in vivo cell labels in genetically-engineered mice. The protocol described in this chapter provides a guideline for the isolation of viable murine epidermal cells expressing β-gal, which can then be subjected to further characterization in vivo or in vitro. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 6 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 2 | 33% |
| Student > Ph. D. Student | 1 | 17% |
| Student > Postgraduate | 1 | 17% |
| Unknown | 2 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 4 | 67% |
| Unknown | 2 | 33% |