| Chapter title |
Clostridium difficile
|
|---|---|
| Chapter number | 18 |
| Book title |
Clostridium difficile
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-6361-4_18 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6359-1, 978-1-4939-6361-4
|
| Authors |
Auchtung, Jennifer M, Robinson, Catherine D, Farrell, Kylie, Britton, Robert A, Jennifer M. Auchtung, Catherine D. Robinson, Kylie Farrell, Robert A. Britton |
| Editors |
Adam P. Roberts, Peter Mullany |
| Abstract |
The commensal microbiome plays an important role in the dynamics of Clostridium difficile infection. In this chapter, we describe minibioreactor arrays (MBRAs), an in vitro cultivation system that we developed that allows for C. difficile physiology to be assayed in the presence of complex fecal microbial communities. The small size of the bioreactors within the MBRAs allows for dozens of reactors to be run simultaneously and therefore several different variables can be tested with limited time and cost. When coupled with experiments in animal models of C. difficile infection, MBRAs can provide important insights into C. difficile physiology and pathogenesis. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 25% |
| Unknown | 3 | 75% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 75% |
| Practitioners (doctors, other healthcare professionals) | 1 | 25% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 2% |
| Unknown | 50 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 12 | 24% |
| Other | 6 | 12% |
| Researcher | 5 | 10% |
| Student > Bachelor | 4 | 8% |
| Professor > Associate Professor | 4 | 8% |
| Other | 11 | 22% |
| Unknown | 9 | 18% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 13 | 25% |
| Biochemistry, Genetics and Molecular Biology | 10 | 20% |
| Immunology and Microbiology | 8 | 16% |
| Unspecified | 1 | 2% |
| Chemical Engineering | 1 | 2% |
| Other | 5 | 10% |
| Unknown | 13 | 25% |