| Chapter title |
Clostridium difficile
|
|---|---|
| Chapter number | 15 |
| Book title |
Clostridium difficile
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-6361-4_15 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6359-1, 978-1-4939-6361-4
|
| Authors |
Johanesen, Priscilla, Lyras, Dena, Priscilla Johanesen, Dena Lyras |
| Editors |
Adam P. Roberts, Peter Mullany |
| Abstract |
Horizontal gene transfer by mobile genetic elements plays an important role in the evolution of bacteria, allowing them to rapidly acquire new traits, including antibiotic resistance. Mobile genetic elements such as conjugative and mobilizable transposons make up a considerable part of the C. difficile genome. While sequence analysis has identified a large number of these elements, experimental analysis is required to demonstrate mobility and function. This chapter describes the experimental methods utilized for determining function and transfer of mobile genetic elements in C. difficile including detection of the circular transfer intermediate and the analysis and confirmation of mobile genetic element transfer to recipient cells. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 25% |
| Unknown | 3 | 75% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 75% |
| Practitioners (doctors, other healthcare professionals) | 1 | 25% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 7 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 2 | 29% |
| Professor | 1 | 14% |
| Unspecified | 1 | 14% |
| Professor > Associate Professor | 1 | 14% |
| Student > Bachelor | 1 | 14% |
| Other | 0 | 0% |
| Unknown | 1 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Immunology and Microbiology | 2 | 29% |
| Agricultural and Biological Sciences | 1 | 14% |
| Unspecified | 1 | 14% |
| Physics and Astronomy | 1 | 14% |
| Medicine and Dentistry | 1 | 14% |
| Other | 0 | 0% |
| Unknown | 1 | 14% |