| Chapter title |
Clostridium difficile
|
|---|---|
| Chapter number | 10 |
| Book title |
Clostridium difficile
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-6361-4_10 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6359-1, 978-1-4939-6361-4
|
| Authors |
Shen, Aimee, Fimlaid, Kelly A, Pishdadian, Keyan, Aimee Shen, Kelly A. Fimlaid, Keyan Pishdadian |
| Editors |
Adam P. Roberts, Peter Mullany |
| Abstract |
The Gram-positive nosocomial pathogen Clostridium difficile induces sporulation during growth in the gastrointestinal tract. Sporulation is necessary for this obligate anaerobe to form metabolically dormant spores that can resist antibiotic treatment, survive exit from the mammalian host, and transmit C. difficile infections. In this chapter, we describe a method for inducing C. difficile sporulation in vitro. This method can be used to study sporulation and maximize spore purification yields for a number of C. difficile strain backgrounds. We also describe procedures for visualizing spore formation using phase-contrast microscopy and for quantifying the efficiency of sporulation using heat resistance as a measure of functional spore formation. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Practitioners (doctors, other healthcare professionals) | 1 | 50% |
| Members of the public | 1 | 50% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 19 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 7 | 37% |
| Student > Bachelor | 2 | 11% |
| Professor | 2 | 11% |
| Other | 1 | 5% |
| Student > Master | 1 | 5% |
| Other | 2 | 11% |
| Unknown | 4 | 21% |
| Readers by discipline | Count | As % |
|---|---|---|
| Immunology and Microbiology | 6 | 32% |
| Biochemistry, Genetics and Molecular Biology | 4 | 21% |
| Agricultural and Biological Sciences | 3 | 16% |
| Chemical Engineering | 1 | 5% |
| Physics and Astronomy | 1 | 5% |
| Other | 0 | 0% |
| Unknown | 4 | 21% |