| Chapter title |
Clostridium difficile
|
|---|---|
| Chapter number | 21 |
| Book title |
Clostridium difficile
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-6361-4_21 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6359-1, 978-1-4939-6361-4
|
| Authors |
Lancaster, Catherine, Rustandi, Richard R, Pannizzo, Paola, Ha, Sha, Catherine Lancaster, Richard R. Rustandi, Paola Pannizzo, Sha Ha |
| Editors |
Adam P. Roberts, Peter Mullany |
| Abstract |
High-performance size-exclusion chromatography (HPSEC or SEC) is a method that can be applied to measure size distribution of proteins, including aggregates, monomers, and fragments. In the biopharmaceutical industry the quantitation of aggregates contained in biotherapeutics and protein-based vaccines is critical given the potential impact on safety, immunogenicity, and efficacy. Hence, aggregation analysis of therapeutic proteins or protein-based vaccine products is almost always a requirement of regulatory agencies. SEC, also referred to as gel-filtration chromatography, separates molecules by size through a porous resin stationary phase. Under isocratic flow small molecules are retained on the column longer than large molecules. Here we describe the use of this SEC technique to characterize aggregation levels for four different protein antigens for a Clostridium difficile vaccine. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Practitioners (doctors, other healthcare professionals) | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 12 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 4 | 33% |
| Other | 1 | 8% |
| Professor | 1 | 8% |
| Student > Ph. D. Student | 1 | 8% |
| Student > Master | 1 | 8% |
| Other | 3 | 25% |
| Unknown | 1 | 8% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 3 | 25% |
| Biochemistry, Genetics and Molecular Biology | 2 | 17% |
| Agricultural and Biological Sciences | 1 | 8% |
| Environmental Science | 1 | 8% |
| Physics and Astronomy | 1 | 8% |
| Other | 3 | 25% |
| Unknown | 1 | 8% |