Chapter title |
Clostridium difficile
|
---|---|
Chapter number | 7 |
Book title |
Clostridium difficile
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-6361-4_7 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6359-1, 978-1-4939-6361-4
|
Authors |
Péchiné, Séverine, Denève-Larrazet, Cécile, Collignon, Anne, Séverine Péchiné, Cécile Denève-Larrazet, Anne Collignon |
Editors |
Adam P. Roberts, Peter Mullany |
Abstract |
Clostridium difficile is responsible for a large spectrum of intestinal diseases ranging from mild diarrhea to fatal colitis depending on the one hand on the strain virulence and on the other on the host. The pathogenesis of C. difficile infection could be seen as a three-step process that takes place after disruption of the digestive microbiota by antibiotics: (1) contamination by and germination of spores; (2) multiplication of vegetative cells in the colonic niche using colonization factors; (3) production of the two toxins TcdA and TcdB and for some strains an additional toxin, the binary toxin CDT. Several studies have been performed to characterize the bacterial factors involved in the colonization step and particularly adhesins.Here, we describe first the methods used to study C. difficile adherence in vitro to epithelial cells and in vivo in animal model intestinal tract, and second the methods used to demonstrate the adhesive properties of surface proteins using Cwp66, GroEL, and FbpA as examples. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 2 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Practitioners (doctors, other healthcare professionals) | 1 | 50% |
Members of the public | 1 | 50% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 18 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Professor | 3 | 17% |
Other | 2 | 11% |
Student > Bachelor | 2 | 11% |
Researcher | 2 | 11% |
Student > Ph. D. Student | 1 | 6% |
Other | 2 | 11% |
Unknown | 6 | 33% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 4 | 22% |
Agricultural and Biological Sciences | 3 | 17% |
Immunology and Microbiology | 2 | 11% |
Veterinary Science and Veterinary Medicine | 1 | 6% |
Physics and Astronomy | 1 | 6% |
Other | 0 | 0% |
Unknown | 7 | 39% |