Assessment of Basic Biological Functions Exerted by miRNAs.
Methods in molecular biology, January 2017
John Nolan, Raymond L. Stallings, Olga Piskareva
Assessment of cell viability and proliferation under different miRNA expression levels is an important step in the evaluation of basic miRNA functional effects within the cell. Here, we describe the overexpression of miRNA in question in cells achieved by transfection with subsequent examination of cell viability and proliferation over a period of time using the acid phosphatase assay.
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