Chapter title |
Profiling the MicroRNA Payload of Exosomes Derived from Ex Vivo Primary Colorectal Fibroblasts.
|
---|---|
Chapter number | 11 |
Book title |
MicroRNA Profiling
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6524-3_11 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6522-9, 978-1-4939-6524-3
|
Authors |
Rahul Bhome BSc MRCS, Rebecca Goh MMed Sci, Karen Pickard PhD, Massimiliano Mellone PhD, A. Emre Sayan PhD, Alex Mirnezami PhD FRCS, Rahul Bhome, Rebecca Goh, Karen Pickard, Massimiliano Mellone, A. Emre Sayan, Alex Mirnezami |
Editors |
Sweta Rani |
Abstract |
The tumor microenvironment is a heterogeneous and dynamic network that exists between cancer and stroma, playing a critical role in cancer progression. Certain tumorigenic signals such as microRNAs are derived from the stroma and conveyed to cancer cells (and vice versa) in nanoparticles called exosomes. Their identification and characterization is an important step in better understanding cellular cross talk and its consequences. To this end we describe how to culture primary ex vivo derived fibroblasts from colorectal tissue, isolate their exosomes, extract exosomal RNA and perform microRNA profiling. |
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