| Chapter title |
MicroRNA Profiling of Exosomes.
|
|---|---|
| Chapter number | 5 |
| Book title |
MicroRNA Profiling
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6524-3_5 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6522-9, 978-1-4939-6524-3
|
| Authors |
Melissa Daly, Lorraine O’Driscoll, Daly, Melissa, O'Driscoll, Lorraine |
| Editors |
Sweta Rani |
| Abstract |
Exosomes are nano-sized membrane-bound vesicles released by a range of different cell types. Exosomes have been shown to specifically package certain membrane and cytosolic proteins and nucleic acids. Furthermore, it has been shown that their contents can be transferred to secondary cells, affecting the recipient cells' cellular processes. Exosomes are present in a multitude of body fluids and so represent a novel source of circulating biomarkers. Here, we describe ultracentrifugation methods suitable for the isolation of exosomes from serum and plasma. We also detail transmission electron microscopy, nanoparticle tracking analysis, and immunoblotting methods suitable for the characterization of exosomes. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 3 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 67% |
| Scientists | 1 | 33% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 24 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 7 | 29% |
| Student > Ph. D. Student | 6 | 25% |
| Student > Master | 2 | 8% |
| Lecturer | 1 | 4% |
| Unspecified | 1 | 4% |
| Other | 3 | 13% |
| Unknown | 4 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
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| Medicine and Dentistry | 4 | 17% |
| Agricultural and Biological Sciences | 3 | 13% |
| Unspecified | 1 | 4% |
| Engineering | 1 | 4% |
| Other | 0 | 0% |
| Unknown | 5 | 21% |