@niederhuth @sangerinstitute @Lucy_Craymer @Preetika_Rana Sure, And the journalists make that clear. But also notes several other examples, and this https://t.co/tWdYB5uCel
RT @tomphilpott: @niederhuth ... but your snip regards just two of the several examples noted in the piece. The WSJ reporters also cites th…
@niederhuth ... but your snip regards just two of the several examples noted in the piece. The WSJ reporters also cites this Nature Biotech article from @sangerinstitute https://t.co/tWdYB5uCel
RT @AfrahBawazeer: في دراسةمثيرة البحث فيNature Biotechnology https://t.co/f0WRSPPs9z تم الكشف عن“انماط أخرى”من أنظمة CRISPR التي لاتتطلب ق…
RT @AfrahBawazeer: في دراسةمثيرة البحث فيNature Biotechnology https://t.co/f0WRSPPs9z تم الكشف عن“انماط أخرى”من أنظمة CRISPR التي لاتتطلب ق…
RT @AfrahBawazeer: في دراسةمثيرة البحث فيNature Biotechnology https://t.co/f0WRSPPs9z تم الكشف عن“انماط أخرى”من أنظمة CRISPR التي لاتتطلب ق…
@APoetsch @Shohael1 Yes, like this ... https://t.co/XrLqVb0XJp
RT @sanchak74: @Shohael1 @APoetsch @MolecularCell @PatelOncology @circuit_logic And yet again ... https://t.co/RTm6cJf91C Data is here.…
@Shohael1 @APoetsch @MolecularCell @PatelOncology @circuit_logic And yet again ... https://t.co/RTm6cJf91C Data is here... https://t.co/FtDCCZSctT I just pick first 500 reads (101 bp) from what is a huge file 10Gb ERR2720632.96 has no match to anyt
@NotKatesBrother @sangerinstitute @michaelkosicki @siTOOLsBiotech @Capt_Shalini How could one have predicted these? Does this paper not contradict this one.. https://t.co/L72PwYW8wc Or long deletions (42kb) https://t.co/bo7TRKBQLR You see what you want
@NotKatesBrother @michaelkosicki Here you go: https://t.co/Ba8DeZQeBC
@JoshC_L @cecilejanssens Yes, this ... https://t.co/tnIoqB4ICc
RT @AfrahBawazeer: في دراسةمثيرة البحث فيNature Biotechnology https://t.co/f0WRSPPs9z تم الكشف عن“انماط أخرى”من أنظمة CRISPR التي لاتتطلب ق…
RT @AfrahBawazeer: في دراسةمثيرة البحث فيNature Biotechnology https://t.co/f0WRSPPs9z تم الكشف عن“انماط أخرى”من أنظمة CRISPR التي لاتتطلب ق…
RT @AfrahBawazeer: في دراسةمثيرة البحث فيNature Biotechnology https://t.co/f0WRSPPs9z تم الكشف عن“انماط أخرى”من أنظمة CRISPR التي لاتتطلب ق…
RT @AfrahBawazeer: في دراسةمثيرة البحث فيNature Biotechnology https://t.co/f0WRSPPs9z تم الكشف عن“انماط أخرى”من أنظمة CRISPR التي لاتتطلب ق…
RT @AfrahBawazeer: في دراسةمثيرة البحث فيNature Biotechnology https://t.co/f0WRSPPs9z تم الكشف عن“انماط أخرى”من أنظمة CRISPR التي لاتتطلب ق…
RT @AfrahBawazeer: في دراسةمثيرة البحث فيNature Biotechnology https://t.co/f0WRSPPs9z تم الكشف عن“انماط أخرى”من أنظمة CRISPR التي لاتتطلب ق…
RT @AfrahBawazeer: في دراسةمثيرة البحث فيNature Biotechnology https://t.co/f0WRSPPs9z تم الكشف عن“انماط أخرى”من أنظمة CRISPR التي لاتتطلب ق…
RT @AfrahBawazeer: في دراسةمثيرة البحث فيNature Biotechnology https://t.co/f0WRSPPs9z تم الكشف عن“انماط أخرى”من أنظمة CRISPR التي لاتتطلب ق…
RT @AfrahBawazeer: في دراسةمثيرة البحث فيNature Biotechnology https://t.co/f0WRSPPs9z تم الكشف عن“انماط أخرى”من أنظمة CRISPR التي لاتتطلب ق…
RT @AfrahBawazeer: في دراسةمثيرة البحث فيNature Biotechnology https://t.co/f0WRSPPs9z تم الكشف عن“انماط أخرى”من أنظمة CRISPR التي لاتتطلب ق…
RT @AfrahBawazeer: في دراسةمثيرة البحث فيNature Biotechnology https://t.co/f0WRSPPs9z تم الكشف عن“انماط أخرى”من أنظمة CRISPR التي لاتتطلب ق…
RT @AfrahBawazeer: في دراسةمثيرة البحث فيNature Biotechnology https://t.co/f0WRSPPs9z تم الكشف عن“انماط أخرى”من أنظمة CRISPR التي لاتتطلب ق…
في دراسةمثيرة البحث فيNature Biotechnology https://t.co/f0WRSPPs9z تم الكشف عن“انماط أخرى”من أنظمة CRISPR التي لاتتطلب قطع DNA،منها النظام الذي يعتمدفي عمله فقط على قطعRNA وبماأن RNAيبقى في الخلايالفترةمحددةمن الزمن حتى ينتهي عمله،هذاسيسمح بالتحكم في توقي
@rtleenay @circuit_logic @PatelOncology Its only because @michaelkosicki knows where to look does he find https://t.co/XrLqVb0XJp This is happening elsewhere, we cant see observe it. Keep repeating-2014 Chip-seq paper, shows Cas9 binds with 10 mismatches
元の論文はRepair of double-strand breaks induced by CRISPR–Cas9 leads to large deletions and complex rearrangement. Michael Kosicki, Kärt Tomberg & Allan Bradley Nature Biotechnology volume 36, pages 765–771 (2018) https://t.co/38vmBdTTBvです👶
@yogendrabmishra Unfortunately, yes ... https://t.co/RTm6cJf91C If u use an instrument (the eye) to analyze the EM spectrum, you will only see VIBGYOR. Illumina are short reads - you will never get larger insertions..
@Kraigka @OscarALizarazo @antonioregalado Thanks :) On a day that put up such results, https://t.co/Ko00np3HOk confirming @michaelkosicki s https://t.co/XrLqVb0XJp to say something like that is reckless to say the least ... And a story which should s
@VictorGenet @dbikard Also by @michaelkosicki https://t.co/XrLqVb0XJp WGS wont get you many OTs. https://t.co/qrhRUjkunv Long reads like Pacb might be an answer, but it has very low coverage. @rtleenay
2/ There is an entire gamut of biological risks that CRISPR has from off-target effects, immunogenicity concerns and random mutations. Though revolutionary, it is still largely a tool in R&D phase that has to be used with precaution. https://t.co/oxE8
@ThomasCDix One has to wonder how many such anecdotal reports are lying unpublished. @michaelkosicki s paper too btw... https://t.co/XrLqVb0XJp cc @Capt_Shalini
@Lexalytics @PaulBarba_ This epitomizes the wrong app of m/c learning in biology. Ilmn read lengths (150 bp) will only detect small indel (till 60 is reported) A longer indel (@michaelkosicki https://t.co/XrLqVb0XJp ) will never be considered - in trai
RT @MiepBos: @LouiseOFresco @WUR https://t.co/ZUfNblNGxr Letter | Published: 16 July 2018 Repair of double-strand breaks induced by CRISPR…
RT @MiepBos: @LouiseOFresco @WUR https://t.co/ZUfNblNGxr Letter | Published: 16 July 2018 Repair of double-strand breaks induced by CRISPR…
RT @MiepBos: @LouiseOFresco @WUR https://t.co/ZUfNblNGxr Letter | Published: 16 July 2018 Repair of double-strand breaks induced by CRISPR…
RT @MiepBos: @LouiseOFresco @WUR https://t.co/ZUfNblNGxr Letter | Published: 16 July 2018 Repair of double-strand breaks induced by CRISPR…
RT @MiepBos: @LouiseOFresco @WUR https://t.co/ZUfNblNGxr Letter | Published: 16 July 2018 Repair of double-strand breaks induced by CRISPR…
RT @MiepBos: @LouiseOFresco @WUR https://t.co/ZUfNblNGxr Letter | Published: 16 July 2018 Repair of double-strand breaks induced by CRISPR…
@LouiseOFresco @WUR https://t.co/ZUfNblNGxr Letter | Published: 16 July 2018 Repair of double-strand breaks induced by CRISPR–Cas9 leads to large deletions and complex rearrangements Michael Kosicki, Kärt Tomberg & Allan Bradley Nature
@FutureFarmingUK @markbuckingham1 @thetimes Sound science @markbuckingham1 🤓 https://t.co/X9xiuVGjq3
@gwern @salonium only relevant for ex vivo theraphy with edited stem cells/T cells) or stuff like https://t.co/EYIrAJBZ3v https://t.co/hdUbOXxZ5e that is on target. What I mean is that you get double-digit % efficiency in semi-suspicious Chinese embryo edi
@karanrajan @kiranshaw @MIT @ARanganathan72 2/2 The biggest problem is off-targets. My take on why CRISPR will fail in larger genomes -its innately non-specific, else viruses would evade it in a day. https://t.co/M5fHcKW7CH Problem is we do not have the
@sci_tweets_ oje, wo soll man da anfangen? Verbraucher als Deppen hinstellen, die #keineAhnung haben (siehe CNV https://t.co/Vh4px8m6YE), nur wissenschaftliche Arbeiten kommentieren, die ins Konzept passen, aber nicht diese https://t.co/kSO03Twkzc … (und d
@guidopmi @soyshadow @ryhertzberger @nrc https://t.co/SD58bU7CtB repair of double-strand breaks induced by CrIsPr–Cas9 leads to large deletions and complex rearrangements Michael Kosicki, Kärt Tomberg & Allan Bradley
2017年時点では、臨床に持ち込む前に倫理的な問題を研究者はもちろんのこと市民とも話し合って慎重に進めていかないとね、って話だったと思うんだけどいずれにせよ基礎研究の域を抜けるのはまだ難しそう、という認識でいいんかな? https://t.co/9VGxuo0HJD
RT @michaelkosicki: We describe extensive Cas9-induced on-target genomic damage. Much of it missed by standard genotyping methods. Publishe…
@LDiesel22 you see this: "Repair of double-strand breaks induced by CRISPR–Cas9 leads to large deletions and complex rearrangements”. https://t.co/5gXgGZhWbz
RT @cobcm: #Biotecnología | Los errores de la técnica CRISPR. La enzima para cortar el ADN utilizada en esta técnica puede suprimir grandes…
RT @cobcm: #Biotecnología | Los errores de la técnica CRISPR. La enzima para cortar el ADN utilizada en esta técnica puede suprimir grandes…
RT @cobcm: #Biotecnología | Los errores de la técnica CRISPR. La enzima para cortar el ADN utilizada en esta técnica puede suprimir grandes…
RT @NatureBiotech: Repair of double-strand breaks induced by #CRISPR–Cas9 leads to large deletions and complex rearrangements https://t.co/…
RT @cobcm: #Biotecnología | Los errores de la técnica CRISPR. La enzima para cortar el ADN utilizada en esta técnica puede suprimir grandes…
#Biotecnología | Los errores de la técnica CRISPR. La enzima para cortar el ADN utilizada en esta técnica puede suprimir grandes fragmentos de ADN o entremezclarlos. Vía @nature #COBCM #investigación https://t.co/gpc1wquTzB https://t.co/NtY2FH42Vr
@msamyARK @AnthemTrading @iamdimitre @csr1223 @SunAndStormInv Its not trivial for NGS to detect OTs, as this Sanger paper tells you https://t.co/XrLqVb0XJp This seminal 2014 paper Cas9 binds "mis-matches can be as high as 10.. 9 can be consecutive". On
@Kathorine @SumanKGuha An off-target can keep mRNA expression same, but make protein function change. Induce frame shift, misfolding, enzymatic activity change... etc ... Have to sequence for OT detection, and that too is difficult for large deletions. h
@JoaoRod27 this is what I was referring to this morning : crispr/Cas9-induced unwanted deletions and large rearrangements of the genome. Poke @NatureBiotech https://t.co/Btt8jLs6gB
@Perdukistan @vittoriabr @pierangelocecc @marcocappato Quanto a CRISPR, è molto bleeding edge, e qualche issue si comincia a vedere: https://t.co/zOyWMYakWM è entusiasmante ma avanti adagio. Il problema comunque è la diffusione involontaria negli ecosistem
RT @DrLGaynor: @IF91 @techreview Great news! Treatments for neuromuscular and neurodegenerative diseases so badly needed. Sadly, recent saf…
@IF91 @techreview Great news! Treatments for neuromuscular and neurodegenerative diseases so badly needed. Sadly, recent safety concerns (potential carcinogenesis) mean that CRISPR-Cas9 won't be ready for translation into clinical applications for the fore
RT @javisamo: @Shora @eldiarioes Es verdad que hay mucha imprecisión, pero ya que hablamos de rigor, sobre el Glifosato: https://t.co/eNwNR…
RT @PabloGenis: Un estudio demuestra que el uso del sistema CRISPR-Cas9 podría ser devastador para los genomas de manera no prevista y frec…
@BWJones My take on why CRISPR will fail in larger genomes- its innately non-specific, else viruses would evade it in a day. https://t.co/M5fHcKW7CH This was known in 2014, before Wall St induced amnesia struck. We are just not looking https://t.co/IN3wB
Un estudio demuestra que el uso del sistema CRISPR-Cas9 podría ser devastador para los genomas de manera no prevista y frecuente https://t.co/dx1uLVJqAA
If you're looking in the vicinity of the CRISPR target site, you may be missing out on a lot of stuff happening further away in the genome: https://t.co/qa5e3OfSk6 Because that's life. "Nothing is ever what it seems but everything is exactly what it is."
RT @javisamo: @Shora @eldiarioes Es verdad que hay mucha imprecisión, pero ya que hablamos de rigor, sobre el Glifosato: https://t.co/eNwNR…
@Shora @eldiarioes Es verdad que hay mucha imprecisión, pero ya que hablamos de rigor, sobre el Glifosato: https://t.co/eNwNRM7Hxi Y sobre el desastre del CRISPR, con este artículo de Nature basta: https://t.co/iVHv3hnTKL Cabe notar que ya se habían autori
@CMYKGenet *notices your cancer-causing deletion mutations* owo what’s this? https://t.co/Q5mYciGz62
It seems that CRISPR-Cas9 still has a long way to go before it is used. https://t.co/x0OV6rhbXQ
RT @klsywd: Today in @michelmorelab journal club we will be discussing this paper! #CRISPR I'll be live tweeting our discussion - so stay…
RT @Trialect: Trialect has several traineeships on CRISPR–Cas9 genome editing. For those interested, please contact us at support@trialect.…
GENE EDITING: CRISPR-Cas9 can lead to unexpected base deletions and complex rearrangements. https://t.co/7BzWaV2QYs Potential pathogenic consequences?
@NephRodby @Nephro_Sparks https://t.co/SlVAZUs8uQ I am sorry about this...But I'm also with you in hoping that CRISPR will find a way out of the rough
ゲノム編集でDNA消失、新技術の信頼ゆらぐ 英チーム:朝日新聞 https://t.co/oCvQSI5stf
RT @NatureBiotech: Repair of double-strand breaks induced by #CRISPR–Cas9 leads to large deletions and complex rearrangements https://t.co/…
RT @NatureBiotech: Repair of double-strand breaks induced by #CRISPR–Cas9 leads to large deletions and complex rearrangements https://t.co/…
Nice to read through it. https://t.co/zX2cskkxt9
RT @NatureBiotech: Repair of double-strand breaks induced by #CRISPR–Cas9 leads to large deletions and complex rearrangements https://t.co/…
RT @NatureBiotech: Repair of double-strand breaks induced by #CRISPR–Cas9 leads to large deletions and complex rearrangements https://t.co/…
RT @NatureBiotech: Repair of double-strand breaks induced by #CRISPR–Cas9 leads to large deletions and complex rearrangements https://t.co/…
RT @NatureBiotech: Repair of double-strand breaks induced by #CRISPR–Cas9 leads to large deletions and complex rearrangements https://t.co/…
RT @ATCGPartners: Unexpected #CRISPR on-target damages @michaelkosicki et al @sangerinstitute https://t.co/DKW3v84XZe As #HyunjiLee & #JinS…
RT @beatesodeik: @EdgeItn. As expected, feared, and predicted there seem to be off-target effects for CRISPR/Cas9 gene editing too. https:/…
RT @tombergk: Our paper on unanticipated Cas9-directed genomic damage now out in @NatureBiotech. Major congrats to first author @michaelkos…
Repair of double-strand breaks induced by CRISPR–Cas9 leads to large deletions and complex rearrangements https://t.co/Gbn6UOxGLy
RT @Jeroen_vdSluijs: Repair of double-strand breaks induced by CRISPR–Cas9 leads to large deletions and complex rearrangements #CRISPR #wak…
RT @OrenoReSource: ゲノム編集でDNA消失、新技術の信頼ゆらぐ 英チーム:朝日新聞 https://t.co/oCvQSI5stf
ゲノム編集でDNA消失、新技術の信頼ゆらぐ 英チーム:朝日新聞 https://t.co/oCvQSI5stf
#CRISPR-Cas9 -- #Gentherapie? Vorsicht! -- "Off-Target-Effekte" der sogenannten "Genschere" https://t.co/UCb4iqTim0 https://t.co/h0FP7OGQAu https://t.co/Z14uRhULj2
Good to see deeper diagnoses of cas9 effects starting to coming out.
RT @OrenoSource: ゲノム編集でDNA消失、新技術の信頼ゆらぐ 英チーム:朝日新聞デジタル https://t.co/QccUCll8AL オンターゲットサイトに欠失や大規模挿入が生じることがある Nat biotech原著↓ https://t.co/dnY0y…
RT @GaetanBurgio: What an interesting paper that just came out in @natureBiotech from Allan Bradley’s group @tombergk on #CRISPR & on-targe…
RT @evolvwing: This thread is a must read for all people out there doing #CRISPR. My takehome: 1) stop saying "NHEJ-repair of CRISPR/Cas9 i…
RT @evolvwing: This thread is a must read for all people out there doing #CRISPR. My takehome: 1) stop saying "NHEJ-repair of CRISPR/Cas9 i…
RT @evolvwing: This thread is a must read for all people out there doing #CRISPR. My takehome: 1) stop saying "NHEJ-repair of CRISPR/Cas9 i…
RT @NatureBiotech: Repair of double-strand breaks induced by #CRISPR–Cas9 leads to large deletions and complex rearrangements https://t.co/…
RT @GaetanBurgio: What an interesting paper that just came out in @natureBiotech from Allan Bradley’s group @tombergk on #CRISPR & on-targe…
RT @evolvwing: This thread is a must read for all people out there doing #CRISPR. My takehome: 1) stop saying "NHEJ-repair of CRISPR/Cas9 i…
RT @GaetanBurgio: What an interesting paper that just came out in @natureBiotech from Allan Bradley’s group @tombergk on #CRISPR & on-targe…