Chapter title |
Assessment of Post-Vaccination Phagocytic Activation Using Candida albicans Killing Assays
|
---|---|
Chapter number | 21 |
Book title |
Vaccines for Invasive Fungal Infections
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-7104-6_21 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7103-9, 978-1-4939-7104-6
|
Authors |
Wenqing Li, Yan Hu |
Editors |
Markus Kalkum, Margarita Semis |
Abstract |
Candida albicans is an important opportunistic fungal pathogen. It is now the fourth leading cause of nosocomial bloodstream infections and a great threat to the immuncompromised patients attributed to the disseminated candidiasis with the mortality up to 40%. Phagocytic cells are the first line of defense against Candida infections. Antibodies induced by vaccination can effectively enhance the capacities of phagocytosis and killing of neutrophils and macrophages. In this chapter, flow cytometric analysis (FACS) and killing assay by plate culture methods are introduced to evaluate the phagocytosis and killing of strains of Candida albicans opsonized with immune serum obtained from mice vaccinated with yeast and recombinant enolase. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 10 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 2 | 20% |
Student > Ph. D. Student | 2 | 20% |
Other | 1 | 10% |
Student > Doctoral Student | 1 | 10% |
Professor > Associate Professor | 1 | 10% |
Other | 1 | 10% |
Unknown | 2 | 20% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 4 | 40% |
Immunology and Microbiology | 2 | 20% |
Veterinary Science and Veterinary Medicine | 1 | 10% |
Engineering | 1 | 10% |
Unknown | 2 | 20% |