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Plant Argonaute Proteins

Overview of attention for book
Cover of 'Plant Argonaute Proteins'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Plant ARGONAUTEs: Features, Functions, and Unknowns
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    Chapter 2 Analysis of the Uridylation of Both ARGONAUTE-Bound MiRNAs and 5′ Cleavage Products of Their Target RNAs in Plants
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    Chapter 3 In Vitro Formation of Plant RNA-Induced Silencing Complexes Using an Extract of Evacuolated Tobacco Protoplasts
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    Chapter 4 In Vitro Analysis of ARGONAUTE-Mediated Target Cleavage and Translational Repression in Plants
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    Chapter 5 Establishment of an In Vivo ARGONAUTE Reporter System in Plants
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    Chapter 6 Immunoprecipitation and High-Throughput Sequencing of ARGONAUTE-Bound Target RNAs from Plants
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    Chapter 7 Identification of ARGONAUTE/Small RNA Cleavage Sites by Degradome Sequencing
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    Chapter 8 Dissecting the Subnuclear Localization Patterns of Argonaute Proteins and Other Components of the RNA-Directed DNA Methylation Pathway in Plants
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    Chapter 9 Detection of Argonaute 1 Association with Polysomes in Arabidopsis thaliana
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    Chapter 10 Functional Analysis of Arabidopsis ARGONAUTEs in Meiosis and DNA Repair
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    Chapter 11 Isolation and Characterization of ARGONAUTE Mutants in Chlamydomonas
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    Chapter 12 ARGONAUTE Genes in Salvia miltiorrhiza: Identification, Characterization, and Genetic Transformation
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    Chapter 13 Cloning and Characterization of Argonaute Genes in Tomato
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    Chapter 14 Nonradioactive Detection of Small RNAs Using Digoxigenin-Labeled Probes
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    Chapter 15 Nonradioactive Plant Small RNA Detection Using Biotin-Labeled Probes
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    Chapter 16 Computational Analysis of Genome-Wide ARGONAUTE-Dependent DNA Methylation in Plants
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    Chapter 17 Structural and Functional Characterization of Plant ARGONAUTE MID Domains
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    Chapter 18 Identification and Analysis of WG/GW ARGONAUTE-Binding Domains
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    Chapter 19 Identification and Evolutionary Characterization of ARGONAUTE-Binding Platforms
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    Chapter 20 Phylogenetic and Evolutionary Analysis of Plant ARGONAUTES
Attention for Chapter 9: Detection of Argonaute 1 Association with Polysomes in Arabidopsis thaliana
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Chapter title
Detection of Argonaute 1 Association with Polysomes in Arabidopsis thaliana
Chapter number 9
Book title
Plant Argonaute Proteins
Published in
Methods in molecular biology, June 2017
DOI 10.1007/978-1-4939-7165-7_9
Pubmed ID
Book ISBNs
978-1-4939-7164-0, 978-1-4939-7165-7
Authors

Lecampion, Cécile, Lanet, Elodie, Robaglia, Christophe, Cécile Lecampion, Elodie Lanet, Christophe Robaglia

Editors

Alberto Carbonell

Abstract

Argonaute (AGO) proteins play a key role in RNA silencing mechanisms. RNA silencing affects both RNA degradation and translation. The characterization of translation-associated RNA silencing mechanisms and components often requires polysome isolation and analysis. In this chapter, we describe the identification of AGO1 association with polysomes through polysome fractionation on sucrose gradient, preparation of proteins by filtration and concentration, and immunoblotting.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 7 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 2 29%
Researcher 2 29%
Unspecified 1 14%
Professor 1 14%
Student > Master 1 14%
Other 0 0%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 4 57%
Agricultural and Biological Sciences 2 29%
Unspecified 1 14%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 March 2018.
All research outputs
#10,132,403
of 12,680,099 outputs
Outputs from Methods in molecular biology
#4,292
of 8,366 outputs
Outputs of similar age
#192,456
of 265,595 outputs
Outputs of similar age from Methods in molecular biology
#11
of 19 outputs
Altmetric has tracked 12,680,099 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 8,366 research outputs from this source. They receive a mean Attention Score of 2.1. This one is in the 29th percentile – i.e., 29% of its peers scored the same or lower than it.
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We're also able to compare this research output to 19 others from the same source and published within six weeks on either side of this one. This one is in the 31st percentile – i.e., 31% of its contemporaries scored the same or lower than it.