↓ Skip to main content

Plant Argonaute Proteins

Overview of attention for book
Cover of 'Plant Argonaute Proteins'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Plant ARGONAUTEs: Features, Functions, and Unknowns
  3. Altmetric Badge
    Chapter 2 Analysis of the Uridylation of Both ARGONAUTE-Bound MiRNAs and 5′ Cleavage Products of Their Target RNAs in Plants
  4. Altmetric Badge
    Chapter 3 In Vitro Formation of Plant RNA-Induced Silencing Complexes Using an Extract of Evacuolated Tobacco Protoplasts
  5. Altmetric Badge
    Chapter 4 In Vitro Analysis of ARGONAUTE-Mediated Target Cleavage and Translational Repression in Plants
  6. Altmetric Badge
    Chapter 5 Establishment of an In Vivo ARGONAUTE Reporter System in Plants
  7. Altmetric Badge
    Chapter 6 Immunoprecipitation and High-Throughput Sequencing of ARGONAUTE-Bound Target RNAs from Plants
  8. Altmetric Badge
    Chapter 7 Identification of ARGONAUTE/Small RNA Cleavage Sites by Degradome Sequencing
  9. Altmetric Badge
    Chapter 8 Dissecting the Subnuclear Localization Patterns of Argonaute Proteins and Other Components of the RNA-Directed DNA Methylation Pathway in Plants
  10. Altmetric Badge
    Chapter 9 Detection of Argonaute 1 Association with Polysomes in Arabidopsis thaliana
  11. Altmetric Badge
    Chapter 10 Functional Analysis of Arabidopsis ARGONAUTEs in Meiosis and DNA Repair
  12. Altmetric Badge
    Chapter 11 Isolation and Characterization of ARGONAUTE Mutants in Chlamydomonas
  13. Altmetric Badge
    Chapter 12 ARGONAUTE Genes in Salvia miltiorrhiza: Identification, Characterization, and Genetic Transformation
  14. Altmetric Badge
    Chapter 13 Cloning and Characterization of Argonaute Genes in Tomato
  15. Altmetric Badge
    Chapter 14 Nonradioactive Detection of Small RNAs Using Digoxigenin-Labeled Probes
  16. Altmetric Badge
    Chapter 15 Nonradioactive Plant Small RNA Detection Using Biotin-Labeled Probes
  17. Altmetric Badge
    Chapter 16 Computational Analysis of Genome-Wide ARGONAUTE-Dependent DNA Methylation in Plants
  18. Altmetric Badge
    Chapter 17 Structural and Functional Characterization of Plant ARGONAUTE MID Domains
  19. Altmetric Badge
    Chapter 18 Identification and Analysis of WG/GW ARGONAUTE-Binding Domains
  20. Altmetric Badge
    Chapter 19 Identification and Evolutionary Characterization of ARGONAUTE-Binding Platforms
  21. Altmetric Badge
    Chapter 20 Phylogenetic and Evolutionary Analysis of Plant ARGONAUTES
Attention for Chapter 3: In Vitro Formation of Plant RNA-Induced Silencing Complexes Using an Extract of Evacuolated Tobacco Protoplasts
Altmetric Badge

Readers on

mendeley
3 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Chapter title
In Vitro Formation of Plant RNA-Induced Silencing Complexes Using an Extract of Evacuolated Tobacco Protoplasts
Chapter number 3
Book title
Plant Argonaute Proteins
Published in
Methods in molecular biology, June 2017
DOI 10.1007/978-1-4939-7165-7_3
Pubmed ID
Book ISBNs
978-1-4939-7164-0, 978-1-4939-7165-7
Authors

Taichiro Iki, Masayuki Ishikawa, Manabu Yoshikawa

Editors

Alberto Carbonell

Abstract

Small RNA-mediated gene silencing is involved in a variety of biological processes among many eukaryotic organisms. The silencing effector, generally referred to as RNA-induced silencing complex (RISC), comprises an ARGONAUTE (AGO) protein and a small single-stranded guide RNA in its core. RISCs recognize target genes containing sequences complementary to the guide RNA and repress their expression transcriptionally or posttranscriptionally. In vitro systems that recapitulate RISC assembly are useful not only to decipher the molecular mechanisms underlying the assembly process itself but also to dissect the downstream silencing pathways mediated by RISCs. Here, we describe a method for in vitro plant RISC assembly, which relies on an extract of evacuolated protoplasts derived from Nicotiana tabacum BY-2 suspension-cultured cells. In this extract, synthetic duplexes of small RNAs are incorporated into AGO proteins that are synthesized by in vitro translation, and then duplex unwinding and selective strand elimination result in formation of mature RISCs.

Mendeley readers

The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 3 100%

Demographic breakdown

Readers by professional status Count As %
Other 1 33%
Student > Master 1 33%
Unknown 1 33%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 1 33%
Medicine and Dentistry 1 33%
Unknown 1 33%