| Chapter title |
Identification and Evolutionary Characterization of ARGONAUTE-Binding Platforms
|
|---|---|
| Chapter number | 19 |
| Book title |
Plant Argonaute Proteins
|
| Published in |
Methods in molecular biology, June 2017
|
| DOI | 10.1007/978-1-4939-7165-7_19 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7164-0, 978-1-4939-7165-7
|
| Authors |
Joshua T. Trujillo, Rebecca A. Mosher |
| Editors |
Alberto Carbonell |
| Abstract |
ARGONAUTE (AGO) proteins are eukaryotic RNA silencing effectors that interact with their binding partners via short peptide motifs known as AGO hooks. AGO hooks tend to cluster in one region of the protein to create an AGO-binding platform. In addition to the presence of AGO hooks, AGO-binding platforms are intrinsically disordered, contain tandem repeat arrays, and have weak sequence conservation even between close relatives. These characteristics make it difficult to identify and perform evolutionary analysis of these regions. Because of their weak sequence conservation, only a few AGO-binding platforms are characterized, and the evolution of these regions is only poorly understood. In this chapter we describe modules developed for computational identification and evolutionary analysis of AGO-binding platforms, with particular emphasis on understanding evolution of the tandem repeat arrays. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 14 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 2 | 14% |
| Student > Ph. D. Student | 2 | 14% |
| Student > Master | 1 | 7% |
| Professor | 1 | 7% |
| Professor > Associate Professor | 1 | 7% |
| Other | 1 | 7% |
| Unknown | 6 | 43% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 5 | 36% |
| Biochemistry, Genetics and Molecular Biology | 3 | 21% |
| Unknown | 6 | 43% |