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Chapter title |
Dissecting the Subnuclear Localization Patterns of Argonaute Proteins and Other Components of the RNA-Directed DNA Methylation Pathway in Plants
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Chapter number | 8 |
Book title |
Plant Argonaute Proteins
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Published in |
Methods in molecular biology, June 2017
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DOI | 10.1007/978-1-4939-7165-7_8 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7164-0, 978-1-4939-7165-7
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Authors |
Cheng-Guo Duan, Jian-Kang Zhu |
Editors |
Alberto Carbonell |
Abstract |
RNA-directed DNA methylation (RdDM) is a nuclear pathway which is comprised of multiple main and accessory protein components, including two plant-specific DNA-dependent RNA polymerases, Pol IV and Pol V, and argonaute (AGO) proteins. Regulation in the RdDM pathway can be achieved via multiple mechanisms, including the spatial distribution of different RdDM components. Here we describe a protocol for dissecting the subnuclear localization of AGO proteins and other RdDM components, including nuclei extraction from seedlings, slide preparation, and subsequent immunostaining. |
Mendeley readers
The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 8 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 2 | 25% |
Researcher | 2 | 25% |
Student > Postgraduate | 1 | 13% |
Student > Doctoral Student | 1 | 13% |
Unknown | 2 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 25% |
Agricultural and Biological Sciences | 2 | 25% |
Nursing and Health Professions | 1 | 13% |
Chemistry | 1 | 13% |
Unknown | 2 | 25% |