Chapter title |
Identification of Ochratoxin A-Producing Black Aspergilli from Grapes Using Loop-Mediated Isothermal Amplification (LAMP) Assays
|
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Chapter number | 22 |
Book title |
Mycotoxigenic Fungi
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6707-0_22 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6705-6, 978-1-4939-6707-0
|
Authors |
Michelangelo Storari, Giovanni A. L. Broggini, Storari, Michelangelo, Broggini, Giovanni A. L. |
Abstract |
The loop-mediated isothermal amplification (LAMP) allows the rapid and specific amplification of target DNA under isothermal conditions without a prior DNA purification step. Moreover, successful amplifications can be directly evaluated through a color change of the reaction solutions. Here, we describe two LAMP assays for the detection of ochratoxin-A producing black aspergilli isolated from grapes. The two assays can detect DNA of OTA-producing black aspergilli following a very simple sample preparation and have the potential to significantly speed up the routine monitoring of these toxigenic molds in vineyards. |
Mendeley readers
Geographical breakdown
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Demographic breakdown
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Student > Bachelor | 2 | 33% |
Researcher | 2 | 33% |
Student > Master | 1 | 17% |
Unknown | 1 | 17% |
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Unknown | 1 | 17% |