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Mycotoxigenic Fungi

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Cover of 'Mycotoxigenic Fungi'

Table of Contents

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    Book Overview
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    Chapter 1 Mycotoxins: An Underhand Food Problem
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    Chapter 2 Alternaria Species and Their Associated Mycotoxins
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    Chapter 3 Aspergillus Species and Their Associated Mycotoxins
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    Chapter 4 Fusarium Species and Their Associated Mycotoxins
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    Chapter 5 Penicillium Species and Their Associated Mycotoxins
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    Chapter 6 Targeting Conserved Genes in Alternaria Species
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    Chapter 7 Targeting Conserved Genes in Aspergillus Species
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    Chapter 8 Targeting Conserved Genes in Fusarium Species
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    Chapter 9 Targeting Conserved Genes in Penicillium Species
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    Chapter 10 Targeting Aflatoxin Biosynthetic Genes
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    Chapter 11 Targeting Trichothecene Biosynthetic Genes
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    Chapter 12 Targeting Ochratoxin Biosynthetic Genes
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    Chapter 13 Targeting Fumonisin Biosynthetic Genes
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    Chapter 14 Targeting Other Mycotoxin Biosynthetic Genes
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    Chapter 15 Evaluating Aflatoxin Gene Expression in Aspergillus Section Flavi
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    Chapter 16 Evaluating Fumonisin Gene Expression in Fusarium verticillioides
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    Chapter 17 Multiplex Detection of Aspergillus Species
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    Chapter 18 Multiplex Detection of Fusarium Species
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    Chapter 19 Multiplex Detection of Toxigenic Penicillium Species
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    Chapter 20 PCR-RFLP for Aspergillus Species
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    Chapter 21 PCR ITS-RFLP for Penicillium Species and Other Genera
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    Chapter 22 Identification of Ochratoxin A-Producing Black Aspergilli from Grapes Using Loop-Mediated Isothermal Amplification (LAMP) Assays
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    Chapter 23 Detection of Transcriptionally Active Mycotoxin Gene Clusters: DNA Microarray
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    Chapter 24 Mycotoxins: A Fungal Genomics Perspective
Attention for Chapter 12: Targeting Ochratoxin Biosynthetic Genes
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Chapter title
Targeting Ochratoxin Biosynthetic Genes
Chapter number 12
Book title
Mycotoxigenic Fungi
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6707-0_12
Pubmed ID
Book ISBNs
978-1-4939-6705-6, 978-1-4939-6707-0
Authors

Antonia Gallo, Giancarlo Perrone

Abstract

The pathway of ochratoxin A (OTA) biosynthesis has not yet been completely elucidated. Essentially, two kind of genes have been demonstrated to be involved in the biosynthesis of OTA. One of them is the nrps gene encoding a non-ribosomal peptide synthetase (NRPS) which catalyzes the ligation between the isocoumarin group, constituting the polyketide group of OTA molecule, and the amino acid phenylalanine.Here we describe a conventional PCR method developed for the detection of OTA-producing molds belonging to Penicillium and Aspergillus genera by Luque et al. (Food Control 29:270-278, 2013). This method is based on the OTA nrps gene of Penicillium nordicum. It produces a specific amplicon of 459 bp and its functionality in naturally infected samples was also demonstrated.

Mendeley readers

The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 7 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 1 14%
Student > Bachelor 1 14%
Researcher 1 14%
Student > Master 1 14%
Unknown 3 43%
Readers by discipline Count As %
Agricultural and Biological Sciences 2 29%
Biochemistry, Genetics and Molecular Biology 1 14%
Medicine and Dentistry 1 14%
Unknown 3 43%