↓ Skip to main content

Mycotoxigenic Fungi

Overview of attention for book
Cover of 'Mycotoxigenic Fungi'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Mycotoxins: An Underhand Food Problem
  3. Altmetric Badge
    Chapter 2 Alternaria Species and Their Associated Mycotoxins
  4. Altmetric Badge
    Chapter 3 Aspergillus Species and Their Associated Mycotoxins
  5. Altmetric Badge
    Chapter 4 Fusarium Species and Their Associated Mycotoxins
  6. Altmetric Badge
    Chapter 5 Penicillium Species and Their Associated Mycotoxins
  7. Altmetric Badge
    Chapter 6 Targeting Conserved Genes in Alternaria Species
  8. Altmetric Badge
    Chapter 7 Targeting Conserved Genes in Aspergillus Species
  9. Altmetric Badge
    Chapter 8 Targeting Conserved Genes in Fusarium Species
  10. Altmetric Badge
    Chapter 9 Targeting Conserved Genes in Penicillium Species
  11. Altmetric Badge
    Chapter 10 Targeting Aflatoxin Biosynthetic Genes
  12. Altmetric Badge
    Chapter 11 Targeting Trichothecene Biosynthetic Genes
  13. Altmetric Badge
    Chapter 12 Targeting Ochratoxin Biosynthetic Genes
  14. Altmetric Badge
    Chapter 13 Targeting Fumonisin Biosynthetic Genes
  15. Altmetric Badge
    Chapter 14 Targeting Other Mycotoxin Biosynthetic Genes
  16. Altmetric Badge
    Chapter 15 Evaluating Aflatoxin Gene Expression in Aspergillus Section Flavi
  17. Altmetric Badge
    Chapter 16 Evaluating Fumonisin Gene Expression in Fusarium verticillioides
  18. Altmetric Badge
    Chapter 17 Multiplex Detection of Aspergillus Species
  19. Altmetric Badge
    Chapter 18 Multiplex Detection of Fusarium Species
  20. Altmetric Badge
    Chapter 19 Multiplex Detection of Toxigenic Penicillium Species
  21. Altmetric Badge
    Chapter 20 PCR-RFLP for Aspergillus Species
  22. Altmetric Badge
    Chapter 21 PCR ITS-RFLP for Penicillium Species and Other Genera
  23. Altmetric Badge
    Chapter 22 Identification of Ochratoxin A-Producing Black Aspergilli from Grapes Using Loop-Mediated Isothermal Amplification (LAMP) Assays
  24. Altmetric Badge
    Chapter 23 Detection of Transcriptionally Active Mycotoxin Gene Clusters: DNA Microarray
  25. Altmetric Badge
    Chapter 24 Mycotoxins: A Fungal Genomics Perspective
Attention for Chapter 6: Targeting Conserved Genes in Alternaria Species
Altmetric Badge

Citations

dimensions_citation
11 Dimensions

Readers on

mendeley
5 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Chapter title
Targeting Conserved Genes in Alternaria Species
Chapter number 6
Book title
Mycotoxigenic Fungi
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6707-0_6
Pubmed ID
Book ISBNs
978-1-4939-6705-6, 978-1-4939-6707-0
Authors

Miguel Ángel Pavón, Inés María López-Calleja, Isabel González, Rosario Martín, Teresa García

Abstract

Real-time polymerase chain reaction (PCR) is a molecular biology technique based on the detection of the fluorescence produced by a reporter molecule, which increases as the reaction proceeds proportionally to the accumulation of the PCR product within each amplification cycle. The fluorescent reporter molecules include dyes that bind to the double-stranded DNA (i.e., SYBR(®) Green) or sequence-specific probes (i.e., Molecular Beacons or TaqMan(®) Probes). Real-time PCR provides a tool for accurate and sensitive quantification of target fungal DNA. Here, we describe a TaqMan real-time PCR method for specific detection and quantification of Alternaria spp. The method uses Alternaria-specific primers and probe, targeting the internal transcribed spacer regions ITS1 and ITS2 of the rRNA gene, and a positive amplification control based on 18S rRNA gene.

Mendeley readers

The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 5 100%

Demographic breakdown

Readers by professional status Count As %
Other 3 60%
Lecturer 1 20%
Student > Master 1 20%
Readers by discipline Count As %
Agricultural and Biological Sciences 1 20%
Computer Science 1 20%
Immunology and Microbiology 1 20%
Medicine and Dentistry 1 20%
Engineering 1 20%
Other 0 0%