| Chapter title |
Evaluating Fumonisin Gene Expression in Fusarium verticillioides
|
|---|---|
| Chapter number | 16 |
| Book title |
Mycotoxigenic Fungi
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6707-0_16 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6705-6, 978-1-4939-6707-0
|
| Authors |
Valeria Scala, Ivan Visentin, Francesca Cardinale, Scala, Valeria, Visentin, Ivan, Cardinale, Francesca |
| Abstract |
Transcript levels of key genes in a biosynthetic pathway are often taken as a proxy for metabolite production. This is the case of FUM1, encoding the first dedicated enzyme in the metabolic pathway leading to the production of the mycotoxins Fumonisins by fungal species belonging to the genus Fusarium. FUM1 expression can be quantified by different methods; here, we detail a protocol based on quantitative reverse transcriptase polymerase chain reaction (RT-qPCR), by which relative or absolute transcript abundance can be estimated in Fusaria grown in vitro or in planta. As very seldom commercial kits for RNA extraction and cDNA synthesis are optimized for fungal samples, we developed a protocol tailored for these organisms, which stands alone but can be also easily integrated with specific reagents and kits commercially available. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 4 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 1 | 25% |
| Professor > Associate Professor | 1 | 25% |
| Lecturer > Senior Lecturer | 1 | 25% |
| Unknown | 1 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 3 | 75% |
| Unknown | 1 | 25% |