↓ Skip to main content

Mycotoxigenic Fungi

Overview of attention for book
Cover of 'Mycotoxigenic Fungi'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Mycotoxins: An Underhand Food Problem
  3. Altmetric Badge
    Chapter 2 Alternaria Species and Their Associated Mycotoxins
  4. Altmetric Badge
    Chapter 3 Aspergillus Species and Their Associated Mycotoxins
  5. Altmetric Badge
    Chapter 4 Fusarium Species and Their Associated Mycotoxins
  6. Altmetric Badge
    Chapter 5 Penicillium Species and Their Associated Mycotoxins
  7. Altmetric Badge
    Chapter 6 Targeting Conserved Genes in Alternaria Species
  8. Altmetric Badge
    Chapter 7 Targeting Conserved Genes in Aspergillus Species
  9. Altmetric Badge
    Chapter 8 Targeting Conserved Genes in Fusarium Species
  10. Altmetric Badge
    Chapter 9 Targeting Conserved Genes in Penicillium Species
  11. Altmetric Badge
    Chapter 10 Targeting Aflatoxin Biosynthetic Genes
  12. Altmetric Badge
    Chapter 11 Targeting Trichothecene Biosynthetic Genes
  13. Altmetric Badge
    Chapter 12 Targeting Ochratoxin Biosynthetic Genes
  14. Altmetric Badge
    Chapter 13 Targeting Fumonisin Biosynthetic Genes
  15. Altmetric Badge
    Chapter 14 Targeting Other Mycotoxin Biosynthetic Genes
  16. Altmetric Badge
    Chapter 15 Evaluating Aflatoxin Gene Expression in Aspergillus Section Flavi
  17. Altmetric Badge
    Chapter 16 Evaluating Fumonisin Gene Expression in Fusarium verticillioides
  18. Altmetric Badge
    Chapter 17 Multiplex Detection of Aspergillus Species
  19. Altmetric Badge
    Chapter 18 Multiplex Detection of Fusarium Species
  20. Altmetric Badge
    Chapter 19 Multiplex Detection of Toxigenic Penicillium Species
  21. Altmetric Badge
    Chapter 20 PCR-RFLP for Aspergillus Species
  22. Altmetric Badge
    Chapter 21 PCR ITS-RFLP for Penicillium Species and Other Genera
  23. Altmetric Badge
    Chapter 22 Identification of Ochratoxin A-Producing Black Aspergilli from Grapes Using Loop-Mediated Isothermal Amplification (LAMP) Assays
  24. Altmetric Badge
    Chapter 23 Detection of Transcriptionally Active Mycotoxin Gene Clusters: DNA Microarray
  25. Altmetric Badge
    Chapter 24 Mycotoxins: A Fungal Genomics Perspective
Attention for Chapter 14: Targeting Other Mycotoxin Biosynthetic Genes
Altmetric Badge

Citations

dimensions_citation
11 Dimensions

Readers on

mendeley
4 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Chapter title
Targeting Other Mycotoxin Biosynthetic Genes
Chapter number 14
Book title
Mycotoxigenic Fungi
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6707-0_14
Pubmed ID
Book ISBNs
978-1-4939-6705-6, 978-1-4939-6707-0
Authors

María J. Andrade, Mar Rodríguez, Juan J. Córdoba, Alicia Rodríguez

Abstract

Real-time PCR (qPCR) methods are adequate tools for sensitive and rapid detection and quantification of toxigenic molds contaminating food commodities. Methods of qPCR for quantifying zearalenone (ZEA)-, sterigmatocystin (ST)-, cyclopiazonic acid (CPA)-, and patulin (PAT)-producing molds have been designed on the basis of specific target genes involved in the biosynthesis of these mycotoxins. In this chapter reliable qPCR protocols to detect and quantify such toxigenic molds are described. All of these methods are suitable when working with mold pure cultures and mold contaminated foods. For ZEA-producing molds, two qPCR using the SYBR Green fluorochrome and based on two polyketide synthase (PKS) genes are detailed. qPCR protocols relied on the fluG and the idh genes able to quantify ST- and PAT-producing molds, respectively, which can be performed by both SYBR Green and TaqMan methodologies are described. Regarding CPA-producing molds a TaqManq PCR method including a competitive internal amplification control is detailed. Since DNA extraction is a critical step in the detection and quantification of toxigenic molds by qPCR, a protocol for extracting DNA from mold pure cultures and food is also described.

Mendeley readers

The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 4 100%

Demographic breakdown

Readers by professional status Count As %
Professor 1 25%
Student > Master 1 25%
Researcher 1 25%
Professor > Associate Professor 1 25%
Readers by discipline Count As %
Veterinary Science and Veterinary Medicine 1 25%
Unspecified 1 25%
Agricultural and Biological Sciences 1 25%
Immunology and Microbiology 1 25%