| Chapter title |
Multiplex Detection of Aspergillus Species
|
|---|---|
| Chapter number | 17 |
| Book title |
Mycotoxigenic Fungi
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6707-0_17 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6705-6, 978-1-4939-6707-0
|
| Authors |
Pedro Martínez-Culebras, María Victoria Selma, Rosa Aznar, Martínez-Culebras, Pedro, Selma, María Victoria, Aznar, Rosa |
| Abstract |
Multiplex real-time polymerase chain reaction (PCR) provides a fast and accurate DNA-based tool for the simultaneous amplification of more than one target sequence in a single reaction. Here a duplex real-time PCR assay is described for the simultaneous detection of Aspergillus carbonarius and members of the Aspergillus niger aggregate, which are the main responsible species for ochratoxin A (OTA) contamination in grapes. This single tube reaction targets the beta-ketosynthase and the acyl transferase domains of the polyketide synthase of A. carbonarius and the A. niger aggregate, respectively.Besides, a rapid and efficient fungi DNA extraction procedure is described suitable to be applied in wine grapes. It includes a pulsifier equipment to remove conidia from grapes which prevents releasing of PCR inhibitors. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 9 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Unspecified | 1 | 11% |
| Lecturer > Senior Lecturer | 1 | 11% |
| Other | 1 | 11% |
| Student > Doctoral Student | 1 | 11% |
| Student > Master | 1 | 11% |
| Other | 1 | 11% |
| Unknown | 3 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 2 | 22% |
| Biochemistry, Genetics and Molecular Biology | 1 | 11% |
| Unspecified | 1 | 11% |
| Immunology and Microbiology | 1 | 11% |
| Medicine and Dentistry | 1 | 11% |
| Other | 0 | 0% |
| Unknown | 3 | 33% |