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Mycotoxigenic Fungi

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Cover of 'Mycotoxigenic Fungi'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Mycotoxins: An Underhand Food Problem
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    Chapter 2 Alternaria Species and Their Associated Mycotoxins
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    Chapter 3 Aspergillus Species and Their Associated Mycotoxins
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    Chapter 4 Fusarium Species and Their Associated Mycotoxins
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    Chapter 5 Penicillium Species and Their Associated Mycotoxins
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    Chapter 6 Targeting Conserved Genes in Alternaria Species
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    Chapter 7 Targeting Conserved Genes in Aspergillus Species
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    Chapter 8 Targeting Conserved Genes in Fusarium Species
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    Chapter 9 Targeting Conserved Genes in Penicillium Species
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    Chapter 10 Targeting Aflatoxin Biosynthetic Genes
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    Chapter 11 Targeting Trichothecene Biosynthetic Genes
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    Chapter 12 Targeting Ochratoxin Biosynthetic Genes
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    Chapter 13 Targeting Fumonisin Biosynthetic Genes
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    Chapter 14 Targeting Other Mycotoxin Biosynthetic Genes
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    Chapter 15 Evaluating Aflatoxin Gene Expression in Aspergillus Section Flavi
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    Chapter 16 Evaluating Fumonisin Gene Expression in Fusarium verticillioides
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    Chapter 17 Multiplex Detection of Aspergillus Species
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    Chapter 18 Multiplex Detection of Fusarium Species
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    Chapter 19 Multiplex Detection of Toxigenic Penicillium Species
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    Chapter 20 PCR-RFLP for Aspergillus Species
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    Chapter 21 PCR ITS-RFLP for Penicillium Species and Other Genera
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    Chapter 22 Identification of Ochratoxin A-Producing Black Aspergilli from Grapes Using Loop-Mediated Isothermal Amplification (LAMP) Assays
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    Chapter 23 Detection of Transcriptionally Active Mycotoxin Gene Clusters: DNA Microarray
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    Chapter 24 Mycotoxins: A Fungal Genomics Perspective
Attention for Chapter 17: Multiplex Detection of Aspergillus Species
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Chapter title
Multiplex Detection of Aspergillus Species
Chapter number 17
Book title
Mycotoxigenic Fungi
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6707-0_17
Pubmed ID
Book ISBNs
978-1-4939-6705-6, 978-1-4939-6707-0
Authors

Pedro Martínez-Culebras, María Victoria Selma, Rosa Aznar

Abstract

Multiplex real-time polymerase chain reaction (PCR) provides a fast and accurate DNA-based tool for the simultaneous amplification of more than one target sequence in a single reaction. Here a duplex real-time PCR assay is described for the simultaneous detection of Aspergillus carbonarius and members of the Aspergillus niger aggregate, which are the main responsible species for ochratoxin A (OTA) contamination in grapes. This single tube reaction targets the beta-ketosynthase and the acyl transferase domains of the polyketide synthase of A. carbonarius and the A. niger aggregate, respectively.Besides, a rapid and efficient fungi DNA extraction procedure is described suitable to be applied in wine grapes. It includes a pulsifier equipment to remove conidia from grapes which prevents releasing of PCR inhibitors.

Mendeley readers

The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 7 100%

Demographic breakdown

Readers by professional status Count As %
Unspecified 1 14%
Researcher 1 14%
Other 1 14%
Student > Doctoral Student 1 14%
Student > Master 1 14%
Other 0 0%
Unknown 2 29%
Readers by discipline Count As %
Unspecified 1 14%
Biochemistry, Genetics and Molecular Biology 1 14%
Agricultural and Biological Sciences 1 14%
Immunology and Microbiology 1 14%
Medicine and Dentistry 1 14%
Other 0 0%
Unknown 2 29%