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Chapter title |
Evaluating Aflatoxin Gene Expression in Aspergillus Section Flavi
|
---|---|
Chapter number | 15 |
Book title |
Mycotoxigenic Fungi
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6707-0_15 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6705-6, 978-1-4939-6707-0
|
Authors |
Paula Cristina Azevedo Rodrigues, Jéssica Gil-Serna, M. Teresa González-Jaén, Rodrigues, Paula Cristina Azevedo, Gil-Serna, Jéssica, González-Jaén, M. Teresa |
Abstract |
The determination of aflatoxin production ability and differentiation of aflatoxigenic strains can be assessed by monitoring the expression of one or several key genes using reverse transcription polymerase chain reaction (RT-PCR). We herein describe the methods for RNA induction, extraction, and quality determination, and the RT-PCR conditions used to evaluate the ability of a given Aspergillus strain to produce aflatoxins. |
Mendeley readers
The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 12 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Other | 2 | 17% |
Researcher | 2 | 17% |
Student > Ph. D. Student | 1 | 8% |
Student > Bachelor | 1 | 8% |
Student > Master | 1 | 8% |
Other | 1 | 8% |
Unknown | 4 | 33% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 3 | 25% |
Immunology and Microbiology | 2 | 17% |
Biochemistry, Genetics and Molecular Biology | 1 | 8% |
Medicine and Dentistry | 1 | 8% |
Unknown | 5 | 42% |