Chapter title |
Targeting Conserved Genes in Fusarium Species
|
---|---|
Chapter number | 8 |
Book title |
Mycotoxigenic Fungi
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6707-0_8 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6705-6, 978-1-4939-6707-0
|
Authors |
Jéssica Gil-Serna, Belén Patiño, Miguel Jurado, Salvador Mirete, Covadonga Vázquez, M. Teresa González-Jaén, Gil-Serna, Jéssica, Patiño, Belén, Jurado, Miguel, Mirete, Salvador, Vázquez, Covadonga, González-Jaén, M. Teresa |
Abstract |
Fumonisins are important mycotoxins contaminating foods and feeds which are mainly produced by F. verticillioides and F. proliferatum. Additionally, both are pathogens of maize and other cereals. We describe two highly sensitive, rapid, and species-specific PCR protocols which enable detection and discrimination of these closely related species in cereal flour or grain samples. The specific primer pairs of these assays were based on the intergenic spacer region of the multicopy rDNA unit which highly improves the sensitivity of the PCR assay in comparison with single-copy target regions. |
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