Chapter title |
Assessment of Functional Norovirus Antibody Responses by Blocking Assay in Mice.
|
---|---|
Chapter number | 13 |
Book title |
Vaccine Design
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3387-7_13 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3385-3, 978-1-4939-3387-7
|
Authors |
Maria Malm, Kirsi Tamminen, Vesna Blazevic |
Editors |
Sunil Thomas |
Abstract |
Norovirus (NoV)-specific serum antibodies bind to NoV-derived virus-like particles (VLPs) and block the binding of VLPs to the host cell attachment factors/receptors, histo-blood group antigens (HBGAs). Blocking antibodies in human sera have been associated with a protection from NoV infection and disease. Studies of experimental NoV VLP-based vaccines measure blocking antibodies in animal sera instead of a traditional virus neutralization assay. This chapter describes the methodology for analyzing blocking antibodies from NoV GII.4 VLP-immunized mouse sera. Protocol for obtaining mouse NoV GII.4-specific immune sera is described, followed by the detailed protocol for blocking assay using synthetic HBGAs. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 6 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 2 | 33% |
Other | 2 | 33% |
Student > Postgraduate | 1 | 17% |
Unknown | 1 | 17% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 3 | 50% |
Chemical Engineering | 1 | 17% |
Agricultural and Biological Sciences | 1 | 17% |
Immunology and Microbiology | 1 | 17% |