Chapter title |
The Use of Microwave-Assisted Solid-Phase Peptide Synthesis and Click Chemistry for the Synthesis of Vaccine Candidates Against Hookworm Infection.
|
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Chapter number | 36 |
Book title |
Vaccine Design
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Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3387-7_36 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3385-3, 978-1-4939-3387-7
|
Authors |
Abdullah A. H. Ahmad Fuaad, Mariusz Skwarczynski, Istvan Toth |
Editors |
Sunil Thomas |
Abstract |
A protein-based vaccine approach against hookworm infection has failed to deliver the expected outcome, due to a problem with an allergic response in the patient or difficulties in the proteins' production. This implication could be overcome by using a chemically synthesized peptide-based vaccine approach. This approach utilizes minimal pathogenic components that are necessary for the stimulation of the immune response without triggering adverse side effects. To boost the peptide's immunogenicity, a lipid core peptide (LCP) system can be utilized as a carrier molecule/immunostimulant. This chapter describes in detail the synthesizing of protected lipoamino acid, the self-adjuvanting moiety (LCP core), the peptide epitope, and the final vaccine candidate. The subunit peptide and the LCP core were synthesized using microwave-assisted solid-phase peptide synthesis (SPPS). Then the final hookworm vaccine construct was assembled using the copper-catalyzed azide-alkyne cycloaddition, or "click," reaction. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 12 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 3 | 25% |
Researcher | 3 | 25% |
Student > Postgraduate | 2 | 17% |
Student > Master | 1 | 8% |
Unknown | 3 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Chemistry | 4 | 33% |
Biochemistry, Genetics and Molecular Biology | 1 | 8% |
Agricultural and Biological Sciences | 1 | 8% |
Mathematics | 1 | 8% |
Medicine and Dentistry | 1 | 8% |
Other | 1 | 8% |
Unknown | 3 | 25% |