Chapter title |
Development of a SARS Coronavirus Vaccine from Recombinant Spike Protein Plus Delta Inulin Adjuvant.
|
---|---|
Chapter number | 14 |
Book title |
Vaccine Design
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3387-7_14 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3385-3, 978-1-4939-3387-7
|
Authors |
Clifton McPherson, Richard Chubet, Kathy Holtz, Yoshikazu Honda-Okubo, Dale Barnard, Manon Cox, Nikolai Petrovsky, McPherson, Clifton, Chubet, Richard, Holtz, Kathy, Honda-Okubo, Yoshikazu, Barnard, Dale, Cox, Manon, Petrovsky, Nikolai |
Editors |
Sunil Thomas |
Abstract |
Given periodic outbreaks of fatal human infections caused by coronaviruses, development of an optimal coronavirus vaccine platform capable of rapid production is an ongoing priority. This chapter describes the use of an insect cell expression system for rapid production of a recombinant vaccine against severe acute respiratory syndrome coronavirus (SARS). Detailed methods are presented for expression, purification, and release testing of SARS recombinant spike protein antigen, followed by adjuvant formulation and animal testing. The methods herein described for rapid development of a highly protective SARS vaccine are equally suited to rapid development of vaccines against other fatal human coronavirus infections, e.g., the MERS coronavirus. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 5 | 19% |
United Kingdom | 2 | 8% |
Venezuela, Bolivarian Republic of | 1 | 4% |
Chile | 1 | 4% |
Unknown | 17 | 65% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 23 | 88% |
Scientists | 2 | 8% |
Practitioners (doctors, other healthcare professionals) | 1 | 4% |
Mendeley readers
Geographical breakdown
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Unknown | 74 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 11 | 15% |
Student > Bachelor | 11 | 15% |
Student > Master | 8 | 11% |
Student > Ph. D. Student | 7 | 9% |
Other | 4 | 5% |
Other | 11 | 15% |
Unknown | 22 | 30% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 14 | 19% |
Medicine and Dentistry | 9 | 12% |
Immunology and Microbiology | 5 | 7% |
Veterinary Science and Veterinary Medicine | 4 | 5% |
Nursing and Health Professions | 3 | 4% |
Other | 13 | 18% |
Unknown | 26 | 35% |